Fig. 1

Intrahost variant frequency analysis of strains tested. Intrahost variant (iSNVs) frequency analysis of four strain tested. distributed across the ZIKV CDS (a). iSNV frequency analysis of passage-specific mutations (b–h). The sum of novel variants not found in ZIKV-6C, ZIKV-6V and ZIKV-6A, which are unique to those observed in ZIKV-I (i). Genetic divergence (FST) of ZIKV-6C, ZIKV-6V and ZIKV-6A was compared with ZIKV-I (j)