Fig. 2From: Circular RNA ciRS-7 affects the propagation of Cryptosporidium parvum in HCT-8 cells by sponging miR-1270 to activate the NF-κB signaling pathwayCiRS-7 acted as a sponge for miR-1270 in HCT-8 cells during C. parvum infection. a Time-dependent expression of ciRS-7 levels in HCT-8 cells during C. parvum infection. b The miR-1270 levels in HCT-8 cells transfected with pcDNA3.1( +)-ciRS-7 plasmid or si-ciRS-7 and exposed to C. parvum infection for 24 h. c The luciferase activity in HCT-8 cells co-transfected with miR-1270 mimics and pmirGLO-ciRS-7-WT or pmirGLO-ciRS-7-MUT. Left represents the putative binding site between ciRS-7 and miR-1270 predicted by starBase v2.0, and right represents the luciferase reporter assay for ciRS-7-WT or ciRS-7-MUT in HCT-8 cells co-transfected with miR-1270 mimics. The data represent the mean ± SD for three independent experiments. *P < 0.05, **P < 0.01Back to article page