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Fig. 3 | Parasites & Vectors

Fig. 3

From: Circular RNA ciRS-7 affects the propagation of Cryptosporidium parvum in HCT-8 cells by sponging miR-1270 to activate the NF-κB signaling pathway

Fig. 3

RelA was a direct target of miR-1270 in HCT-8 cells following C. parvum infection. a Time-dependent expression of relA mRNA levels in HCT-8 cells during C. parvum infection. b Protein levels of RELA in HCT-8 cells transfected with miR-1270 mimics or inhibitor and exposed to C. parvum infection for 24 h. c The luciferase activity in HCT-8 cells co-transfected with miR-1270 mimics and pmirGLO-relA-WT or pmirGLO-relA-MUT. Left represents the putative binding site between the 3′-UTR of relA and miR-1270 predicted by starBase v2.0, and right represents the luciferase reporter assay for relA-WT or relA-MUT in HCT-8 cells co-transfected with miR-1270 mimics. d The mRNA levels of relA in HCT-8 cells transfected with miR-1270 mimics or inhibitor and exposed to C. parvum infection for 24 h. The data represent the mean ± SD for three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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