Fig. 3

Phenotypes produced by RNAi of CpCHSA in the pupal stage (n ≥ 50). siCHSA treatment in the 0- to 1-h pupae. a CpCHSA protein levels were determined using western blotting analysis with CpCHSA-specific polyclonal antibodies at 24 h after injection of siCHSA. b qPCR analysis of CpCHSA gene expression at 24 h after the injection of siCHSA. c siCHSA injection into pupae reduced the eclosion rate. d The survival rate of PE mosquitoes. All surviving individuals were used for measurement. Data represent three biological replicates (50 individuals in each replicate) with three technical replicates, and the results are shown as the mean ± SEM. Asterisks indicate a significant difference at *P < 0.05, **P < 0.01, ***P < 0.001, according to Student’s t-test