Fig. 5

Effect of CpCHSA on chitin metabolism of the new and old chitin. a RNAi of CpCHSA in the pupal stage (n ≥ 50) The red arrow indicates the position of the collected samples. The chitin staining experiment in the integument was performed by injecting siCHSA or NC into the pupae after 12 h (b) and 24 h (c). N-chitin New chitin, O-chitin old chitin