Fig. 8

Phenotypes produced by RNAi of CpCHSA in the adult stage. The 12- to 24-h PE mosquitoes were injected with siCHSA (300 ng per mosquito) or NC. a At the 72-h PE stage, CpCHSA protein levels were determined using western blotting analysis with a CpCHSA-specific polyclonal antibody. b qPCR analysis of CpCHSA gene expression level at the 72-h PE stage. c, e Injection of siCHSA had no effect on the adult phenotype. d, f Kaplan–Meier survival curves were used to determine the female and male adult survival rates. The results are shown as the mean ± SEM. Asterisks indicate a significant difference at ***P < 0.001, according to Student’s t-test