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Table 1 Molecular approaches for the detection of Wolbachia in vectors and onchocercid nematodes

From: Wolbachia: endosymbiont of onchocercid nematodes and their vectors

Primer name Gene targeted Type of PCR Product size References
99F, 99R 16S rRNA cPCR 895 [50, 164]
Wspecf, Wspecr 16S rRNA cPCR 438 [165]
16SWolbf, 16SWolbr 16S rRNA cPCR 1014 [34]
INTF1, INTR 16S rRNA cPCR 130 [166]
INTF2, INTR2 16S rRNA cPCR 136 [166]
553F_W, 1334R_W 16S rRNA cPCR 781 [54]
WolbF, Wspecr 16S rRNA cPCR   [167]
63f, 1387R, 76f, 1012R 16S rRNA Nested PCR 852 [168]
WN16S-F, WN16S-R 16S rRNA qPCR   [169]
W-Specf, W-Specr, 16S rRNA qPCR 438 [170]
W-Specf, W16S 102
WSPintF, WSPintR wsp cPCR 576 [34, 114, 168]
81F, 691R wsp cPCR 610 [51, 171]
136 F, 691R wsp (Group A) 556
308 F, 691R wsp (Subgroup Mel) 405
328 F, 691R wsp (Subgroup AlbA) 379
173F, 691R wsp (Subgroup Mel and AlbA) 541
181F, 691R wsp (Subgroup wPap) 506
165F, 691R wsp (Subgroup wAus) 506
81F, 531R wsp (Subgroup wPap and wAus) 460
81 F, 522R wsp (Group B) 442
183F, 691R wsp (Subgroup Pip) 501
wspF, wspR, grF, grR wsp qPCR   [172]
wspTMF, wspTMR wsp qPCR   [59]
WSP.F3, WSP.B3, WSP.FIP, WSP.BIP wsp LAMP assay   [173]
FIP_wMel/wPop wsp (wMel/ wPop) LAMP assay   [59]
BIP_wMel/wPop
F3_wMel/wPop
B3_wMel/wPop
LpF_wMel/wPop
LpB_wMel/wPop
ftsZ_F1, ftsZ_R1 FtsZ cPCR 524 [40]
FtsZUniF, FtsZUniR FtsZ cPCR   [174]
ftsZfl, ftsZrl FtsZ cPCR 1043–1055 [38]
Wol1F, Wol1R, Wol7F, Wol7R FtsZ Nested PCR 147 [175]
MLST primers 16 s rRNA, gatB, FtsZ, hcpA, fbpA
coxA. wsp
MLST   [40]
ftsZ 291, ftsZ 477 FtsZ qPCR   [176]
WSP 420, WSP 583 wsp
Bm-wFtsZ-F, BmwFtsZ-R FtsZ qPCR   [96]
groEL-F, groEL-R groEL cPCR   [32]
WgroF1, WgroRev1 groEL cPCR 873 [35]
WgltAF1, WgltARev1 Citrate synthase (gltA) cPCR 627 [35]
FbpA_F1, FbpA_R1 FbpA cPCR 509 [40]
hcpA_F1, hcpA_R1 HcpA cPCR 516 [40]
coxA_F1, coxA_R1 coxA cPCR 487 [40]
COIintF, COIintR COI cPCR 689 [34]
Wseq01F, Wseq02R gatB cPCR 471 [31]