Table 1 Molecular approaches for the detection of Wolbachia in vectors and onchocercid nematodes

99F, 99R

16S rRNA

cPCR

895

[50, 164]

Wspecf, Wspecr

16S rRNA

cPCR

438

[165]

16SWolbf, 16SWolbr

16S rRNA

cPCR

1014

[34]

INTF1, INTR

16S rRNA

cPCR

130

[166]

INTF2, INTR2

16S rRNA

cPCR

136

[166]

553F_W, 1334R_W

16S rRNA

cPCR

781

[54]

WolbF, Wspecr

16S rRNA

cPCR

[167]

63f, 1387R, 76f, 1012R

16S rRNA

Nested PCR

852

[168]

WN16S-F, WN16S-R

16S rRNA

qPCR

[169]

W-Specf, W-Specr,

16S rRNA

qPCR

438

[170]

W-Specf, W16S

102

WSPintF, WSPintR

wsp

cPCR

576

[34, 114, 168]

81F, 691R

wsp

cPCR

610

[51, 171]

136 F, 691R

wsp (Group A)

556

308 F, 691R

wsp (Subgroup Mel)

405

328 F, 691R

wsp (Subgroup AlbA)

379

173F, 691R

wsp (Subgroup Mel and AlbA)

541

181F, 691R

wsp (Subgroup wPap)

506

165F, 691R

wsp (Subgroup wAus)

506

81F, 531R

wsp (Subgroup wPap and wAus)

460

81 F, 522R

wsp (Group B)

442

183F, 691R

wsp (Subgroup Pip)

501

wspF, wspR, grF, grR

wsp

qPCR

[172]

wspTMF, wspTMR

wsp

qPCR

[59]

WSP.F3, WSP.B3, WSP.FIP, WSP.BIP

wsp

LAMP assay

[173]

FIP_wMel/wPop

wsp (wMel/ wPop)

LAMP assay

[59]

BIP_wMel/wPop

F3_wMel/wPop

B3_wMel/wPop

LpF_wMel/wPop

LpB_wMel/wPop

ftsZ_F1, ftsZ_R1

FtsZ

cPCR

524

[40]

FtsZUniF, FtsZUniR

FtsZ

cPCR

[174]

ftsZfl, ftsZrl

FtsZ

cPCR

1043–1055

[38]

Wol1F, Wol1R, Wol7F, Wol7R

FtsZ

Nested PCR

147

[175]

MLST primers

16 s rRNA, gatB, FtsZ, hcpA, fbpA

coxA. wsp

MLST

[40]

ftsZ 291, ftsZ 477

FtsZ

qPCR

[176]

WSP 420, WSP 583

wsp

Bm-wFtsZ-F, BmwFtsZ-R

FtsZ

qPCR

[96]

groEL-F, groEL-R

groEL

cPCR

[32]

WgroF1, WgroRev1

groEL

cPCR

873

[35]

WgltAF1, WgltARev1

Citrate synthase (gltA)

cPCR

627

[35]

FbpA_F1, FbpA_R1

FbpA

cPCR

509

[40]

hcpA_F1, hcpA_R1

HcpA

cPCR

516

[40]

coxA_F1, coxA_R1

coxA

cPCR

487

[40]

COIintF, COIintR

COI

cPCR

689

[34]

Wseq01F, Wseq02R

gatB

cPCR

471

[31]