Fig. 2

Analysis of specific antibodies by ELISA and Western blot. a, b BALB/c mice (n = 10) were immunized three times with Trx-His tag (immunization control) and recombinant proteins (Pbg37, PSOP25, Pbg37+PSOP25, and Pbg37-PSOP25). The equal volume sera of every mouse per group were pooled at day 10 after the final immunization for ELISA coated with the recombinant Pbg37 (a) and recombinant PSOP25 (b) polypeptides after removal of the Trx tag. Error bars indicate standard deviation. **P < 0.01 indicates a significant difference between the immunization and control groups (ANOVA). c, d The lysates of P. berghei gametocytes (GC) and ookinete (Ook) at 10 μg per lane were separated by 10% SDS-PAGE and probed with anti- Pbg37+PSOP25 antisera (c) and anti- Pbg37-PSOP25 antisera (d). The protein loading was estimated by the anti-rHsp70 sera