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Fig. 3 | Parasites & Vectors

Fig. 3

From: Evaluation of two sexual-stage antigens as bivalent transmission-blocking vaccines in rodent malaria

Fig. 3

Indirect immunofluorescence analysis using the bivalent immune sera. Parasites of different developmental stages were fixed and permeabilized with 0.1% Triton X-100. They were stained with antisera against Pbg37+PSOP25 (a) and Pbg37-PSOP25 (b) (1:200) as the primary antibodies (green). The parasites were also co-labeled with antibodies against the markers for different stages (red), including α-tubulin (α) for male gametocytes/gametes, P47 for female gametocytes, Pbs21 for zygotes and ookinetes, and SET for the nucleus of gametocytes. Alexa Fluor 488-conjugated goat anti-mouse IgG antibodies and Alexa Fluor 555-conjugated goat anti-rabbit IgG antibodies were used as the secondary antibodies. c Negative controls showing the ookinetes labeled with the Trx-His antisera or with the secondary antibodies only. The nucleus was stained with Hoechst-33258 (blue). Images were obtained under the same conditions at a magnification of ×1000. DIC differential interference contrast microscopy. Scale bar = 5 μm

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