Fig. 3
From: Differential expression of TgMIC1 in isolates of Chinese 1 Toxoplasma with different virulence
SDS-PAGE and western blot analysis of the rTgMIC1 protein. a Expression of pET30a-MIC1/BL21 in small amounts. Lane M: protein marker; lane 1–3: mono-clone picked from conversion plates after induction with IPTG; lane 4: uninduced control. b Large amounts of expression detection. Lane M: protein marker; lane 1: proteins in pET30a-MIC1/BL21 after induction with IPTG; lane 2: supernatant of pET30a-MIC1/BL21 bacteria after ultrasonication; lane 3: precipitation of pET30a-MIC1/BL21 bacteria after ultrasonication; lane 4: uninduced control. c Western blot analysis of the rTgMIC1. Lane M: protein marker; lane 1: rTgMIC1 detected by HIS monoclonal antibodies. d The rTgMIC1 purified by Ni2+-affinity chromatography. Lane M: protein marker; lane 1: 0.5 mg/ml BSA; lane 2: 1.0 mg/ml BSA; lane 3: purified rTgMIC1 proteins