Fig. 4
From: Differential expression of TgMIC1 in isolates of Chinese 1 Toxoplasma with different virulence
Preparation and specific detection of TgMIC1 polyclonal antibody. a The titer of the purified TgMIC1 polyclonal antibody was detected by indirect ELISA. The titer is the dilution corresponding to the minimum OD reading greater than the maximum OD/2. b The purity of TgMIC1 polyclonal antibody indicated by SDS-PAGE. Lane M: protein marker; lane 1: heavy and light chains of the purified TgMIC1 polyclonal antibody. c Different recombinant bacteria from Toxoplasma proteins reacted with the TgMIC1 polyclonal antibody. Lane M: protein marker; lane 1: rTgMIC1; lane 2: pET30a-MIC1 bacteria; lane 3–5: pET30a-MIC4, pET30a-MIC6 and pET28a-ROP18, respectively. d The TgMIC1 polyclonal antibody reacted with proteins from different type of T. gondii strains and parasites. Lane M: protein marker; lane 1–6: RH, TgCtwh3, TgCtwh6, Plasmodium, and Schistosoma, respectively. e Confocal microscopy images showing the localization of TgMIC1 (red) in GFP-RH tachyzoites (green) challenges HFF cells for 24 h. DAPI (blue) was used to stain the nuclei. The scale bars are 10 µm