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Fig. 6 | Parasites & Vectors

Fig. 6

From: Differential expression of TgMIC1 in isolates of Chinese 1 Toxoplasma with different virulence

Fig. 6

TgMIC1 polyclonal antibody regulates parasite invasion and growth. a Representative images of invaded and attached tachyzoites with HFF cells. Infected HFF cells were incubated with TgGAP45 polyclonal antibody and then analyzed by immunofluorescence staining to evaluate the invasion rate of T. gondii. Extracellular (yellow) and intracellular (red) tachyzoites in merged pictures; DAPI (blue) was used to stain the nuclei. Scale bar, 10 μm. b Histogram of T. gondii invasion. The tachyzoites were counted under a microscope (200×). c Microscopy showing the growth of parasites in HFF monocytes. Scale bar, 5 μm. d The percentage of parasites/vacuole histogram; the tachyzoites were counted under a microscope (400). *p < 0.05, ***p < 0.001 versus TgCtwh3 group. Data are represented as mean ± SD for three independent experiments

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