Table 1 Primers and probes used in this study
From: Development of a real-time PCR method for rapid diagnosis of canine babesiosis and anaplasmosis
Target gene | Primer/probe name | Sequence (5’–3’) | Product size | References |
|---|---|---|---|---|
B.canis Bc28.1 | F5’UTR281&2 | AGTCGATACCTCCGAGAATAG | 852 nt | [29] |
R3’UTR281 | CATTACGCCCACAAA TAGTCA |  | [29] | |
A. phagocytophilum mdh | mdh 1fa | GTGTTGCGGGTATCTGTCA | 593 nt | [30] |
mdh 2ra | TCCTCCCTTGCGAGTCCT | Â | [30] | |
B.canis Bc28 | BC28F3 | GCTACGTCCGTTGAAGCC | 65 nt | This study |
BC28R3 | TCAGCGGAATAACGTTCAGC | Â | This study | |
BC28 | Fam–AGCCAGTCGATCTGCTCCTTTAAGCT |  | This study | |
A. phagocytophilum mdh | APmdhF | CAGACTATGCAGCTATTGAGGG | 100 nt | This study |
APmdhR | GCATTAGCCATGAGCAAATCTTC | Â | This study | |
APmdhProbe | Hex–TCCTTTCTAGGAAGGCCTGC |  | This study |