Table 3 Lower limit of detection (LOD) of the RT-PCR assay for B. canis and A. phagocytophilum using 20 intra-assay replicates at two different concentration levels
From: Development of a real-time PCR method for rapid diagnosis of canine babesiosis and anaplasmosis
Gene copies/ reaction | B. canis Bc28 gene | A. phagocytophilum mdh gene | ||
|---|---|---|---|---|
Mean Ct ± SD | LOD, % | Mean Ct ± SD | LOD, % | |
10 | 29.629 ± 1.058 | (20/20) 100% | 32.073 ± 0.997 | (20/20) 100% |
5 | 33.197 ± 1.044 | (18/20) 90% | 34.141 ± 0.522 | (14/20) 70% |