Fig. 2From: Loop-mediated isothermal amplification (LAMP) assays targeting 18S ribosomal RNA genes for identifying P. vivax and P. ovale species and mitochondrial DNA for detecting the genus PlasmodiumLAMP and PCR amplification of serial dilutions of pMD19-Pan, pMD19-Pv and pMD19-Po. a Observation of the green fluorescence by the naked eye under UV light of LAMP amplification from 102 to 109 copies/μl of pMD19-Pan and pMD19-Pv and from 103 to 109 copies/μl of pMD19-Po. b Observation of the white fluorescence image on the Gel imager under UV light of LAMP amplification from 102 to 109 copies/μl of pMD19-Pan and pMD19-Pv and from 103 to 109 copies/μl of pMD19-Po. c Amplification curves of LAMP reactions of amplification from 102 to 109 copies/μl of pMD19-Pan and pMD19-Pv and from 103 to 109 copies/μl of pMD19-Po. d Observation of bands following agarose gel electrophoresis (2% gel) of PCR amplification from 104 to 109 copies/μL of pMD19-Pan, pMD19-Pv and pMD19-Po. ΔRn value is the normalized fluorescence value obtained after deducting the baseline value. M Marker, N negative controlBack to article page