Loop-mediated isothermal amplification (LAMP) assays targeting 18S ribosomal RNA genes for identifying P. vivax and P. ovale species and mitochondrial DNA for detecting the genus Plasmodium

Table 2 Results as diagnosed by microscopic examination, nested PCR and malaria LAMP PAN assay

Plasmodium species	Microscopy (cases, n)	PCR (cases, n)	Malaria Pan LAMP (cases, n)	P. vivax LAMP (cases, n)	P. ovale LAMP (cases, n)
Plasmodium genus			241
P. falciparum	80	92
P. ovale	63	34 P. ovale curtisi, 24 P. ovale wallikeri			57
P. vivax	61	87		87
P. malariae	4	4
Negative	71	38	38	192	222
Total	279	279	279	279	279

Primers	Sensitivity	Specificity	LOD (parasites/μL)
Malaria Pan	100% (95% CI 98.48–100%)	100% (95% CI 90.75–100%)	3.73 ± 0.33
P. vivax	100% (95% CI 95.85–100%)	100% (95% CI 98.1–100%)	3.79 ± 0.71
P. ovale	98.28% (95%CI: 90.76–99.96%)	100% (95% CI 98.34–100%)	4.15 ± 0.36

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