Fig. 3

Localization of mRNAs encoding SmCB1, SmPOP, SmTsp-2, and Sm29 in adult S. mansoni males using FISH. Semi-thin (6 μm) sections of adult S. mansoni male worms were probed with DIG-labelled RNA probes designed to detect SmCB1, SmPOP, SmTsp-2, or Sm29 mRNAs. The probes hybridized with transcripts were visualized by tyramide amplification assay (red). Adult males were monitored in three parts: a a head part, b testes, and c a posterior part with the focus on parenchyma, tegument and gut. DAPI was used to label nuclear DNA (blue). The left columns show merged fluorescent channels; in the right columns, the fluorescent red signal is merged with differential interference contrast. Transcripts of SmPOP, SmTsp-2, and Sm29 were detected in and around the esophagus, in the testes, and in parenchymal cell subtypes. SmPOP and SmTsp-2 were also detected in tegumental cells and in tegumental tubercles. SmCB1 is expressed strongly in the gut, weakly in parenchyma around the esophagus and testes. The scale bars represent 100 µm