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Fig. 4 | Parasites & Vectors

Fig. 4

From: Spatial expression pattern of serine proteases in the blood fluke Schistosoma mansoni determined by fluorescence RNA in situ hybridization

Fig. 4

Localization of mRNA encoding SmSP1 to SmSP5 in adult S. mansoni females using FISH. Semi-thin (6 μm) sections of adult S. mansoni female worms were probed with DIG-labelled RNA probes designed to detect mRNAs of serine proteases SmSP1 to SmSP5. Probes hybridized with transcripts were visualized by tyramide amplification assay (red). Adult females were monitored in three parts: a an anterior part, b oviduct, mature and immature oocytes, and c vitellaria and gut. DAPI was used to label nuclear DNA (blue). The left columns show merged fluorescent channels; in the right columns, the fluorescent red signal is merged with differential interference contrast. a A strong signal of transcripts (red) belonging to SmSP2 and SmSP4 genes and less abundant signal of SmSP1 and SmSP5 was detected in parenchymal cells of the anterior part of the females. SmSP3 was not detected in this part. b Strong fluorescence of SmSP2, SmSP4, and SmSP5 was observed in mature and immature oocytes, while SmSP1 sense transcript is expressed exclusively in mature oocytes, and faint signal of SmSP3 was detected in ovary. SmSP1 and SmSP4 are also expressed in the oviduct. c Transcripts of all studied SmSPs were present in vitellaria but with different patterns of expression. A faint fluorescent signal of SmSP2 and SmSP4 transcripts is also detected in tegument. The scale bars represent 100 µm

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