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Fig. 5 | Parasites & Vectors

Fig. 5

From: Spatial expression pattern of serine proteases in the blood fluke Schistosoma mansoni determined by fluorescence RNA in situ hybridization

Fig. 5

Semi-thin (6 μm) sections of adult S. mansoni male worms were probed with DIG-labelled RNA probes designed to detect mRNAs of serine proteases SmSP1 to SmSP5. Probes hybridized with transcripts were visualized by tyramide amplification assay (red). Adult males were monitored in three parts: a a head part, b testes, and c a posterior part with the focus on parenchyma, tegument, and gut. DAPI was used to label nuclear DNA (blue). The left columns show merged fluorescent channels; in the right columns, the fluorescent red signal is merged with differential interference contrast. a Except for SmSP5, transcripts of all SmSPs were identified in parenchyma around the esophagus. b Strong signal of SmSP1 to SmSP4 transcripts was detected in testes where only weak signal of SmSP5 mRNA was observed. c All SmSPs are present in parenchymal cell subtypes, however each with a different pattern. Compared to others, SmSP4 is abundant in parenchymal cells, in tegumental cytons, and also in tegumental tubercles. The scale bars represent 100 µm

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