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Table 1 Developmental parameters of Culicoides obsoletus (s.l.) subadult life stages under five rearing conditions (RCs)

From: Temperature and food sources influence subadult development and blood-feeding response of Culicoides obsoletus (sensu lato) under laboratory conditions

 

RC1

RC2

RC3

RC4

RC5

Total

Temperature (°C)

28

24

24

20/16 Day/night

20/16 Day/night

 

Nematodes/algae

X

X

X

X

X

 

Dung patches

–

–

X

–

X

 

No. eggs

1905

1748

1852

903

1102

7510

Egg hatching rate (%)

60

87

40

14

8

48

No. hatched eggs

1140

1515

738

129

89

3611

Larval survival ratea (%)

13

15

14

7

16

14

No. larvae

147

224

104

9

14

498

Pupation rate (%)

74

98

91

55.5

86

88.5

No. pupae

109

220

95

5

12

441

Adult emergence rate (%)

39

50

73

40

83

52.5

No. adults

42

109

69

2

10

232

No. ♂ adults

42

83

53

1

5

184

No. ♀ adults

0

26

16

1

5

48

Sex ratio (♂:♀)

1:0

3:1

3:1

1:1

1:1

 

Average overall development time (days)

26

26

21

33

30

 
  1. Culicoides subadult life stages were reared in an incubator under 16/8 h light/dark conditions and 80% relative humidity on 1% liquid broth agar at the indicated temperature and provided with the additional food sources as indicated in the table
  2. aSurvival from first-instar to fourth-instar larvae