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Fig. 2 | Parasites & Vectors

Fig. 2

From: Fasciola gigantica tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes

Fig. 2

The relative expression of CaBP4 in F. gigantica life cycle stages. The messenger RNA (mRNA) transcriptional patterns of CaBP4 in eggs, juvenile [28 and 42 days post-infection (dpi)] and adult (98 dpi) flukes were examined by real-time polymerase chain reaction. The transcription value of CaBP4 in the eggs was normalized to 1.0. The relative changes in the gene transcription ratio were normalized to the transcription of a reference gene and calculated by the 2−ΔΔCt method. The data are presented as mean ± SD from three independent experiments. Data were analyzed by one-way ANOVA. ns Non-significant; ***P < 0.001, ****P < 0.0001

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