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Fig. 3a, b | Parasites & Vectors

Fig. 3a, b

From: Fasciola gigantica tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes

Fig. 3a, b

Western blotting was performed to confirm the expression of recombinant Fg-CaBP4 (rFg-CaBP4) protein [molecular weight protein ladder (M)]. a The recombinant protein expression before isopropyl-ß-D-thiogalactopyranoside induction (0 h), protein expression at different times (2, 4, 6 h) and the purified protein resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (P). b The purified protein was transferred to a polyvinylidene difluoride membrane which was probed with rat sera containing anti-rFg-CaBP4 antibodies (1) or incubated with normal rat sera (2)

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Parasites & Vectors

ISSN: 1756-3305

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