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Fig. 5 | Parasites & Vectors

Fig. 5

From: Involvement of TLR4 signaling regulated-COX2/PGE2 axis in liver fibrosis induced by Schistosoma japonicum infection

Fig. 5

TLR4 signaling was verified to induce hepatic stellate cell activation and fibrogenesis in vitro. a LX-2 was triggered with different doses of LPS for 24 h or was triggered with LPS (100 ng/ml) for indicated time courses. b, c LX-2 cells were pretreated with TAK242 for indicated concentrations for 30 min prior to stimulation with LPS (100 ng/ml) for 24 h. Non-treatment or LPS treatment alone were used as controls. d LX-2 cells were transfected with either control siRNA (NC) or two different TLR4 siRNAs and cultured for 24 h, and then stimulated with LPS (100 ng/ml) for 24 h. Indicated protein expression levels were determined using western blotting as shown in the left panels, and the semi-quantitative results are shown in the right panels. Each experiment was conducted at least twice. β-Tubulin was used as a loading control

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