Fig. 6

Analysis of the invasion and replication ability of eIF-5A knockdown tachyzoites. a Attachment ability of eIF-5A knockdown parasites. RH and eIF-5A knockdown parasites were inoculated in the fixed monolayer cells for 1 h, and immunofluorescence assay (IFA) was performed, after which the number of parasites adhered to host cells was counted. The data are indicative of three individual experiments, * P < 0.05, Student’s t test. b Invasion ability of eIF-5A knockdown parasites. RH and eIF-5A knockdown parasites were inoculated in HFF cells for 1 h, and IFA was performed, after which the number of parasites invading the cells was counted. The data are indicative of three individual experiments, * P < 0.05, Student’s t test. c Replication ability of eIF-5A knockdown tachyzoites. RH and eIF-5A knockdown parasites were inoculated in HFF cells for 24 h, and the parasite number of 100 parasitophorous vacuoles was counted and analyzed using Student’s t test with GraphPad Prism 5.0 (GraphPad Software, USA). The data are indicative of three individual experiments (** P < 0.01)