Fig. 7
Growth of eIF-5A knockdown and RH parasites in vitro and in vivo. a Plaque assay in HFF cells. RH and eIF-5A knockdown parasites were inoculated in HFF cells, and plaques are visible as clear zones against the background of crystal violet-stained HFF monolayers. b Size of the plaques. The size of plaques was evaluated by Image Pro Plus 6.0 (Media Cybernetics, USA), and the data were analyzed by Student’s t test using GraphPad Prism 5.0 (GraphPad Software, USA). The data were indicative of three individual experiments (* P < 0.05). c Virulence analysis of eIF-5A knockdown parasites in BALB/c mice. Two doses of RH and eIF-5A knockdown parasites were injected into mice (200 or 2000 parasites/mouse and 5 mice/dose, respectively). The survival time was monitored each day, and the significance of each dose was compared by the log-rank test and Gehan Breslow Wilcoxon test (*** P < 0.001)