Fig. 3From: The effects of plant cysteine proteinases on the nematode cuticleSEM of WT (Bristol N2) C. elegans after incubation in 1 µM papain or papain + E64 at time points of 10, 15 and 30 min. a The worms were apparently intact and unaffected when incubated with papain + E64 for 30 min, with the alae clearly visible (arrowed yellow). b After 10 min of incubation in papain, the cuticle appeared to be split longitudinally, which became more extensive at 15 min of incubation (c). By 30 min, the cuticle was split longitudinally, with a section of the cuticle totally destroyed or curled in on itself (d). Bar = 25 µmBack to article page