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Fig. 5 | Parasites & Vectors

Fig. 5

From: The effects of plant cysteine proteinases on the nematode cuticle

Fig. 5

SEM images of cpi-2(RB1207, ok1256) C. elegans incubated in papain or papain + E64. a Worms incubated for 30 min in papain + E64 clearly showed the delicate intact structure of the cuticle, with the alae being clearly visible (yellow arrow). b By contrast, after 10 min of incubation, worms incubated in papain showed tearing or were split longitudinally along a line very close to the alae (arrowed red). c At 15 min, the papain-induced cuticle disruption probably caused the cuticles to detach from the rest of the body in what appeared to be sheets of cuticle (arrowed red). d After 30 min of incubation, the cuticle was often split longitudinally, in some cases, with the alae on the opposite side of the worm still intact (yellow arrow). Bar = 10 µm

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