Skip to main content
Fig. 7 | Parasites & Vectors

Fig. 7

From: TBP and SNAP50 transcription factors bind specifically to the Pr77 promoter sequence from trypanosomatid non-LTR retrotransposons

Fig. 7

Mapping the preferential binding sites of rTBP and nuclear proteins to the Pr77 sequence. a Sequence of Pr77 and of the oligo pairs employed as probes for binding assays of b rTBP and c nuclear proteins (NP) of the parasite. In a, the DPE motif is shaded, and introduced point mutations are underlined. A total of 6.35 μM b rTBP or 3 μg of c NP was incubated with 0.33 nM or 100,000 cpm of each dsDNA probe in b and c, respectively at 37 °C for 30 min. All reactions were also carried out in the absence of b rTBP or c NP. Reactions were resolved in 6% polyacrylamide native gels. The black arrowhead indicates the formed complexes of reduced mobility, and the asterisks indicate the electrophoretic mobility of the nucleic acid-free conformations

Back to article page