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Fig. 3 | Parasites & Vectors

Fig. 3

From: Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China

Fig. 3

MLSA of B. miyamotoi Hetao-1 and other relapsing fever borreliae. A phylogenetic inference of the concatenated housekeeping gene sequences of the representative relapsing fever borreliae is shown. The arrow indicates the B. miyamotoi strain Hetao-1 isolated in this study. Consensus sequences for the eight housekeeping genes were isolated from the draft genome sequence of B. miyamotoi strains Hetao-1, trimmed to lengths and concatenated in the order: clpA, clpX, nifS, pepX, pyrG, recG, rplB, and uvrA according to the Borrelia PubMLST database. For phylogenetic reconstruction, the maximum likelihood model based on the Kimura two-parameter model with MEGA 6.0 was used with 1000 bootstrap replicates. Borrelia turcica IST7 was used as outgroup. The ST number designated in each strain indicates the “sequence type” registered in the Borrelia PubMLST database

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