Fig. 6

Analysis of differentially expressed transcripts between stages of Eimeria stiedae. a An UpSet plot showing the sets of differentially expressed transcripts from four different developmental stages, including the quantitative analysis of aggregate intersections between stages. The vertical bars show the number of intersecting transcripts between stages, denoted by the connected black circles below the histogram. The horizontal bars show the transcript set size between stages. b A summary of the numbers of up- and downregulated differentially expressed transcripts. c Validation of RNA-Seq results by quantitative real-time reverse transcription PCR (qRT-PCR). Plot of gene expression (fold change) determined by the RNA-Seq (X-axis) and qRT-PCR (Y-axis) of 60 selected genes (Pearson’s correlation, R² = 0.8763, p < 0.01). The fold change of expression was expressed as log2 values. d Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of differentially expressed transcripts, including cellular processes, environmental information processing, metabolism, and organismal systems