Fig. 7

Transcription throughout the development of Eimeria stiedae. a Heat map showing the transcriptional levels for the genes either specific to a stage or overexpressed in a stage. Based on these, the free-living stages (unsporulated oocysts [Ou] and sporulated oocysts [Os]) cluster separately from the parasitic stages (fourth-generation merozoites [Mz4] and gametocytes [Gc]). b Soft clustering of all genes with non-zero FPKM (fragments per kilobase per million mapped reads) in the four stages was included. Nine clusters associated into five different groups based on the extent of shared genes among them, each characterized primarily by having high level of normalized transcription in one of these four developmental stages. c Cluster overlap panel for gene clusters presented in (d). Genes that did not have > 0.5 membership coefficient in any of the clusters were not assigned to any clusters and are not shown here