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Table 1 Cloning reaction system and conditions of the H. longicornis Pmy gene

From: Evaluation on two types of paramyosin vaccines for the control of Haemaphysalis longicornis infestations in rabbits

Reagent Volume (μl) Temperature (°C) Time Cycle
cDNA (500 ng/μl) 1.2 94 3 min 1 Cycle
2 × Power Taq PCR MasterMix 5 94 30 s 35 Cycles
Forward primer (100 mol/μl) 0.4 56 30 s
Reverse primer (100 mol/μl) 0.4 72 2 min
ddH2O 3 72 10 min 1 Cycle