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Table 1 Cloning reaction system and conditions of the H. longicornis Pmy gene

From: Evaluation on two types of paramyosin vaccines for the control of Haemaphysalis longicornis infestations in rabbits

Reagent

Volume (μl)

Temperature (°C)

Time

Cycle

cDNA (500 ng/μl)

1.2

94

3 min

1 Cycle

2 × Power Taq PCR MasterMix

5

94

30 s

35 Cycles

Forward primer (100 mol/μl)

0.4

56

30 s

Reverse primer (100 mol/μl)

0.4

72

2 min

ddH2O

3

72

10 min

1 Cycle