Fig. 2
DENV load in co-infection by DPI, tissue, and mosquito line. Ae. aegypti were orally challenged with DENV-2 (orange circles) and DENV-3 (navy diamonds) simultaneously, and both viruses were fed to mosquitoes at ~ 3 × 105 DENV genome copies/ml. Mosquito tissues (midgut, carcass, and salivary glands) were collected at nine days post-infection, and DENV RNA load was determined via RT-qPCR using serotype-specific probes. Mosquitoes with undetectable viral RNA load are not represented in this graph. Black bars represent treatment medians. Each symbol represents a single mosquito sample. Total number of mosquitoes screened per day was n = 7 for the wild-type line and n = 30 for wMel