Fig. 4

WT line susceptibility to DENV in co- and mono-infections by DPI, tissue, and serotype. Ae. aegypti mosquitoes were orally challenged with DENV-2 and DENV-3 in both mono- and co-infection experiments. Each bar represents the proportion of mosquitoes positive for either serotype in the mono- (grey) and co-infection (blue) experiments for each day post-infection. Mosquito tissues (midgut, carcass, and salivary glands) were collected at nine days post-infection, and DENV RNA load was determined via RT-qPCR using serotype-specific probes. Sample size n = 7 per day