Skip to main content
Fig. 5 | Parasites & Vectors

Fig. 5

From: Screening and identification of Theileria annulata subtelomere-encoded variable secreted protein-950454 (SVSP454) interacting proteins from bovine B cells

Fig. 5

Screening of interacting proteins with SVSP454 using the Y2H system. a Detection of the autoactivation ability and toxicity of the bait plasmid (pGBKT7-SVSP454) in Y2H Gold cells. b Identification of the transformation efficiency of the pGBKT7-SVSP454 bait plasmid. The colonies were randomly selected and identified by colony PCR using the specific primers for SVSP454. M: DL 2000 DNA marker; 1–18: colonies grown on SDO plates with SVSP454 bait plasmid. c Screening for the putative prey proteins interacting with the SVSP454 bait plasmid. The cotransformants of bait and prey plasmids were successively grown on DDO/X/A and QDO/X/A plates. The SVSP950454 bait and pGBKT7 empty plasmids were transformed into Y2H Gold cells and cultured on different plates. Negative control (pGADT7-T and pGBKT7-Lam) plasmids and positive control (pGADT7-T and pGBKT7-53) plasmids were cotransformed into Y2H Gold cells and incubated on DDO and DDO/X/A plates at 30 °C

Back to article page

Parasites & Vectors

ISSN: 1756-3305

Contact us