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Fig. 6 | Parasites & Vectors

Fig. 6

From: Screening and identification of Theileria annulata subtelomere-encoded variable secreted protein-950454 (SVSP454) interacting proteins from bovine B cells

Fig. 6

Expression and subcellular localization of SVSP454 and its prey proteins (CCDC181 and MRPL30) in HEK293T cells. SVSP454 (4 µg) and its potential interacting proteins-CCDC181 (4 µg) and MRPL30 (4 µg) were individually a or pairwise (SVSP454 (2 µg)-CCDC181 (2 µg) and SVSP454 (2 µg)-MRPL30 (2 µg)), b transfected into HEK293T cells, stained with a mouse anti-MYC tag monoclonal antibody or rabbit anti-FLAG tag monoclonal antibody, and incubated with a goat anti-mouse Alexa Fluor 488- or donkey anti-rabbit 594-conjugated antibody. Cell nucleus was stained with Hoechst 33342

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Parasites & Vectors

ISSN: 1756-3305

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