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Fig. 8 | Parasites & Vectors

Fig. 8

From: Screening and identification of Theileria annulata subtelomere-encoded variable secreted protein-950454 (SVSP454) interacting proteins from bovine B cells

Fig. 8

SVSP454 binds to both CCDC181 and MRPL30 in the cell context. a Schematic of recombinant plasmid construction for the BiFC assays. VN and VC were fused to the C-terminus of SVSP454 and its interacting proteins (CCDC181 and MRPL30), and each fusion protein also contained a FLAG or HA tag at its N-terminus. b SVSP454-VN, SVSP454-VC, CCDC181-VN, CCDC181-VC, MRPL30-VN, MRPL30-VC, SVSP454-VN-CCDC181-VC, SVSP454-VN-MRPL30-VC SVSP454-VC-CCDC181-VN and SVSP454-VC-MRPL30-VN were expressed individually or pairwise in HEK293T cells as indicated. Four micrograms of plasmids were transfected into HEK293T cells. The MFI of BiFC fluorescent signals was detected by flow cytometry and is presented as MFI values relative to the signal of the control HEK293T cells. Error bars represent the SD from three independent BiFC experiments. c HEK293T cells were cotransfected with the pairs SVSP454-VN-CCDC181-VC (4 μg), SVSP454-VN-MRPL30-VC (4 μg), SVSP454-VC-CCDC181-VN (4 μg) and SVSP454-VC-MRPL30-VN (4 μg). Empty vectors pBiFC-VN173 (4 μg) and pBiFC-VC155 (4 μg) served as the negative control and were individually transfected into HEK293T cells

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Parasites & Vectors

ISSN: 1756-3305

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