Comparison of mitochondrial genome and development of specific PCR primers for identifying two scuticociliates, Pseudocohnilembus persalinus and Uronema marinum

Table 1 Comparison of mitochondrial genomes of P. persalinus and U. marinum

Species	Length (bp)	Entire genome							Protein-coding gene
Species	Length (bp)	A (bp)	T (bp)	C (bp)	G (bp)	N (bp)^a	Genes	GC (%)	Length (aa)	AT (%) (all)	AT (%) (3rd)
Pseudocohnilembus persalinus	38,375	14430	15135	4101	4509	200	40	22.44	9174	77.01	76.18
Uronema marinum	39,845	15869	16404	4562	4010	0	43	19	9929	81.45	85.98

^aThe mitochondrial genome of P. persalinus had two gaps, and the length of gaps was unknown. The number of N in each gap region was in accordance with NCBI sequence submission requirements, and the default was 100
Length (aa): Total length of amino acid sequence of all mitochondrial proteins. “aa” represents amino acids; AT (%) (all): The percentage of the A and T base of the coding region sequence of all proteins in the total coding region sequence; AT (%) (3rd): The percentage of the sequence consisting of the sum of A and T bases in the third base of all amino acid codons

ISSN: 1756-3305