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Table 1 Primers and PCR conditions

From: Correction to: First multilocus sequence typing (MLST) of Giardia duodenalis isolates from humans in Romania

Gene

PCR reaction

Product length (bp)

Primer name

Primer (5'-3')

PCR conditions

References

bg

1st

753

G7

AAGCCCGACGACCTCACCCGCAGTGC

A

[37]

G759

GAGGCCGCCCTGGATCTTCGAGACGAC

2nd

511

B-F

GAACGAACGAGATCGAGGTCCG

B

B-R

CTCGACGAGCTTCGTGTT

gdh

1st

n.g

GDHeF

TCAACGTYAAYCGYGGYTTCCGT

A

[37]

GDHeR

GTTRTCCTTGCACATCTCC

2nd

432

GDHiF

CAGTACAACTCYGCTCTCGG

C

  

GDHiR

GTTRTCCTTGCACATCTCC

ITS1

1st

347

FW1

TGGAGGAAGGAGAAGTCGTAAC

D

[16]

RV1

GGGCGTACTGATATGCTTAAGT

2nd

315

FW2

AAGGTATCCGTAGGTGAACCTG

 

RV2

ATATGCTTAAGTTCCGCCCGTC

tpi

1st

605

ALA3542

AAATIATGCCTGCTCGTCG

A

[38]

ALA3542

CAAACCTTITCCGCAAACC

2nd

530

ALA3544

CCCTTCATCGGIGGTAACTT

 

ALA3545

GTGGCCACCACICCCGTGCC

  1. PCR conditions: A (1 cycle: 95 °C for 5 min; 40 cycles: 95 °C for 45 s, 50 °C for 30 s, 72 °C for 60 s; 1 cycle: 72 °C for 7 min); B (1 cycle: 95 °C for 5 min; 35 cycles: 95 °C for 45 s, 55 °C for 30 s, 72 °C for 45 s; 1 cycle: 72 °C for 7 min); C (1 cycle: 95 °C for 5 min; 40 cycles: 95 °C for 45 s, 60 °C for 30 s, 72 °C for 45 s; 1 cycle: 72 °C for 7 min); D (1 cycle: 95 °C for 5 min; 35 cycles: 95 °C for 30 s, 59 °C for 30 s, 72 °C for 30 s; 1 cycle: 72 °C for 7 min)