Gene
|
PCR reaction
|
Product length (bp)
|
Primer name
|
Primer (5'-3')
|
PCR conditions
|
References
|
---|
bg
|
1st
|
753
|
G7
|
AAGCCCGACGACCTCACCCGCAGTGC
|
A
|
[37]
|
G759
|
GAGGCCGCCCTGGATCTTCGAGACGAC
|
2nd
|
511
|
B-F
|
GAACGAACGAGATCGAGGTCCG
|
B
|
B-R
|
CTCGACGAGCTTCGTGTT
|
gdh
|
1st
|
n.g
|
GDHeF
|
TCAACGTYAAYCGYGGYTTCCGT
|
A
|
[37]
|
GDHeR
|
GTTRTCCTTGCACATCTCC
|
2nd
|
432
|
GDHiF
|
CAGTACAACTCYGCTCTCGG
|
C
|
| |
GDHiR
|
GTTRTCCTTGCACATCTCC
|
ITS1
|
1st
|
347
|
FW1
|
TGGAGGAAGGAGAAGTCGTAAC
|
D
|
[16]
|
RV1
|
GGGCGTACTGATATGCTTAAGT
|
2nd
|
315
|
FW2
|
AAGGTATCCGTAGGTGAACCTG
|
|
RV2
|
ATATGCTTAAGTTCCGCCCGTC
|
tpi
|
1st
|
605
|
ALA3542
|
AAATIATGCCTGCTCGTCG
|
A
|
[38]
|
ALA3542
|
CAAACCTTITCCGCAAACC
|
2nd
|
530
|
ALA3544
|
CCCTTCATCGGIGGTAACTT
|
|
ALA3545
|
GTGGCCACCACICCCGTGCC
|
- PCR conditions: A (1 cycle: 95 °C for 5 min; 40 cycles: 95 °C for 45 s, 50 °C for 30 s, 72 °C for 60 s; 1 cycle: 72 °C for 7 min); B (1 cycle: 95 °C for 5 min; 35 cycles: 95 °C for 45 s, 55 °C for 30 s, 72 °C for 45 s; 1 cycle: 72 °C for 7 min); C (1 cycle: 95 °C for 5 min; 40 cycles: 95 °C for 45 s, 60 °C for 30 s, 72 °C for 45 s; 1 cycle: 72 °C for 7 min); D (1 cycle: 95 °C for 5 min; 35 cycles: 95 °C for 30 s, 59 °C for 30 s, 72 °C for 30 s; 1 cycle: 72 °C for 7 min)