Aedes albopictus diversity and relationships in south-western Europe and Brazil by rDNA/mtDNA and phenotypic analyses: ITS-2, a useful marker for spread studies

Table 3 Genetic diversity of Ae. albopictus populations from south-western Europe and Brazil. (a) Based on mtDNA cox1 gene sequences;( b) based on 5.8S-ITS-2 rDNA sequences

(a)
Geographical origin	n	H	Variable sites	Mutations (P-info + singleton sites)	K	Hd ± SD	Nd ± SD (× 10^–2)	Tajima’s D	Fu’s Fs
Valencia	26	5	5	2 + 3	0.59	0.406 ± 0.116	0.042 ± 0.015	− 1.556 (P > 0.10)	− 2.192
Barcelona	14	4	3	2 + 1	0.87	0.495 ± 0.151	0.061 ± 0.020	− 0.244 (P > 0.10)	− 0.728
Mallorca Island	25	6	5	4 + 1	1.55	0.723 ± 0.073	0.110 ± 0.016	0.4958 (P > 0.10)	− 0.619
Perpignan	10	2	5	5 + 0	1.94	0.389 ± 0.164	0.137 ± 0.058	0.2412 (P > 0.10)	3.702
Goiania, Jurujuba Manaus	36	1	–	–	–	–	–	–	–

(b)
Geographical origin	n^a	H	Variable sites^b	Mutations (P-info + singleton sites)	indels	K	Hd ± SD	Nd ± SD (× 10^–2)	Tajima’s D	Fu’s Fs
Valencia	46	34	76	17 + 24	35	3.50	0.905 ± 0.038	0.773 ± 0.119	− 2.155 (P < 0.05)	− 25.061
Barcelona	17	16	32	6 + 15	11	4.22	0.971 ± 0.032	0.885 ± 0.141	− 1.273 (P > 0.10)	− 7.968
Mallorca Island	45	33	103	13 + 24	66	4.73	0.947 ± 0.022	1.120 ± 0.115	− 1.620 (0.10 > P > 0.05)	− 18.034
Perpignan	41	30	115	14 + 18	83	3.59	0.937 ± 0.023	0.885 ± 0.104	− 1.897 (P < 0.05)	− 12.790
Goiania,	50	38	87	17 + 30	40	7.46	0.963 ± 0.015	1.658 ± 0.081	− 1.144 (P > 0.10)	− 17.039
Jurujuba	26	14	83	13 + 3	67	4.56	0.862 ± 0.045	1.078 ± 0.115	0.306 (P > 0.10)	− 0.319
Manaus	28	22	78	14 + 18	46	7.17	0.968 ± 0.024	1.612 ± 0.105	− 0.660 (P > 0.10)	− 10.540

n, samples sequenced; n^a, sequences obtained (cloning + sequencing); ^b, including mutations + sites with alignment gaps. p values are indicated in parentheses
H, nº of haplotypes; P-info, parsimony informative; Indels, insertions/deletions; K, average number of nucleotide differences; Hd, haplotype (gene) diversity; Nd, nucleotide diversity (per site)

ISSN: 1756-3305