Fig. 3

Transcription levels of superoxides, peroxidases, and fumarate reductase were assessed by RT-qPCR in wild-type parasites and FeSOD-A^−/−/+ mutants. DNA polymerase gene (LINF_160021500) was used as constitutive normaliser, and the fold change was calculated by the 2^–∆∆Ct method. Enzymes analysed: iron superoxide dismutase SODA (LINF_080007900—FeSOD-A), iron superoxide dismutase – putative SODB1 (LINF_320024000—FeSOD-B1), iron superoxide dismutase – putative SODB2 (LINF_320024100—FeSOD-B2), superoxide dismutase—putative (SOD LINF_300033000), superoxide dismutase—putative (SOD LINF_320033200) and superoxide dismutase—putative (SOD LINF_340012900), ascorbate peroxidase (LINF_340005600—APX), tryparedoxin peroxidase (LINF_150018600, LINF_150018800, and LINF_150019000—TXNPx), NADH-dependent fumarate reductase (LINF_350013000, LINF_350016600, and LINF_350016700 FRD), peroxiredoxin (LINF_230005400—PRX), type II (glutathione peroxidase-like) tryparedoxin peroxidase (LINF_260013100—type II (GPX-like) TXNPx), glutathione peroxidase—putative (LINF_360038100—GPX). Ordinary one-way ANOVA test with Bonferroni post hoc test was used to compare WT parasites and mutants for each gene separately. * represents significant differences in relation to the wild-type parasite (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p ≤ 0.0001). Pairwise comparisons: FeSODA WT vs. C1 F(2, 19) = 57.84, p < 0.0001; FeSODA WT vs. C2 F(2, 19) = 57.84, p < 0.0001; FeSODB1 and FeSODB2 WT vs. C2 F(2, 21) = 8.596, p = 0.0012; SOD LINF_300033000 WT vs. C2 F(2, 17) = 5.314; p = 0.0147; SOD LINF_340012900 WT vs. C1 F(2, 13) = 4.993, p = 0.0152; APX WT vs. C1 F(2, 11) = 14.44, p = 0.0013; APX WT vs. C2 F(2, 11) = 14.44, p = 0.0058; TXNPx WT vs. C1 F(2, 13) = 4.463, p = 0.0241