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Table 2 Percentage survival and qualitative motility observed of Dirofilaria immitis microfilariae at 6 h and 3 and 5 days after thawing from a cryopreserved state of − 80 °C for > 30 days

From: Cryogenic preservation of Dirofilaria immitis microfilariae, reactivation and completion of the life-cycle in the mosquito and vertebrate hosts

Day post-thaw Survival parameter Cryoprotectant/cryoprotectant combination
5% Glycerol 5% DMSO 6% DMSO 5% Glycerol/PVPa 5% DMSO/PVPa 6% DMSO/PVPa
Day 0 (6 h) Qualitative motility Fast Fast Fast Fast Fast Fast
Day 3 % Survival 36 69 63 61 93 88
Qualitative motility Fast Fast Fast Fast Fast Fast
Day 5 % Survival 30 76 58 73 94 93
Qualitative motility Medium Medium Medium Medium Medium Medium
  1. PVP Polyvinyl pyrolidone
  2. aPVP was added to a final concentration of 4 mM