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Table 3 Adult D. immitis recovery counts from ferrets on Day 133 (and Day 113 as indicated) from microfilariae freshly collected from a dog or cryopreserved

From: Cryogenic preservation of Dirofilaria immitis microfilariae, reactivation and completion of the life-cycle in the mosquito and vertebrate hosts

Treatmenta Animal ID Live worm counts
Males Females Total % Recovery
T01—FCmf C79 10 9 19 76
C88 6 12 18 72
C63b 7 8 15 60
Mean worm counts 7.7 9.7 17.3 69.3
SD 2.1 2.1 2.1 8.3
T02—CPmf C61 8 10 18 72
C64 6 5 11 44
C83b 4 6 10 40
Mean worm counts 6.0 7.0 13.0 52.0
SD 2.0 2.6 4.4 17.4
Percentage reduction of mean worm counts from T01    24.9%  
P-value T01:T02 (two-tailed Student’s t-test)   0.1801  
  1. SD Standard deviation
  2. aAnimals allotted to T01 received L3s derived from mf freshly collected from a dog (FCmf); animals allotted to T02 received L3s derived from mf had been cryopreserved for 4 weeks (CPmf)
  3. bAnimals removed from the study on Day 113