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Fig. 3 | Parasites & Vectors

Fig. 3

From: A study of the composition of the Obsoletus complex and genetic diversity of Culicoides obsoletus populations in Spain

Fig. 3

Validation of the optimized cytochrome c oxidase I (cox1) multiplex polymerase chain reaction assay with an annealing temperature of 60 ºC. Lane L 100-base pair (bp) ladder, lanes 1 and 3 C. scoticus individuals from GC1, lanes 2 and 4 C. obsoletus individuals from GC1, lane C- negative control of the PCR. The multiplex PCR included forward and reverse ObL and ScN primers. For other abbreviations, see Figs. 1 and  2

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