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Table 1 Mosquito feeding rate according the feeding parameters being tested

From: Optimization of the feeding rate of Anopheles farauti s.s. colony mosquitoes in direct membrane feeding assays

Feeding parameters Total number of mosquitoes in cups Total number fed Total unfed Empirical average feeding rate (%)* Range (%) p value**
Starving time
 0 h 916 240 676 27 4–45 < 0.01
 2 h 864 288 576 34 15–56 < 0.01
 4 h 933 368 565 40 20–54 < 0.05
 6 h 954 405 549 45 23–82 < 0.01
 Overnight (~ 21 h) 841 492 349 60 19–97 Ref
Type of starving
 Access to water 217 134 83 62 43–89 0.47
 Dry starving 234 167 67 71 31–92 Ref
Membrane type
 Baudruche 476 389 87 85 70–100  < 0.05
 Parafilm 457 268 189 53 42–76 Ref
Exposure time
 10 min 426 326 100 77 63–98 0.63
 20 min 469 368 101 80 43–100 0.61
 30 min 386 314 72 81 72–89 Ref
Mosquito age
 3 days 631 447 184 75 39–96 0.38
 5 days 606 473 133 81 50–93 Ref
 7 days 613 449 164 75 55–90 0.08
Light/Dark
 Light 317 274 43 85 64–96 0.88
 Dark 326 276 50 84 63–96 Ref
Volume of blood
 125 µL 289 190 99 65 50–88 < 0.05
 250 µL 272 229 43 84 67–96 0.54
 500 µL 295 256 39 87 77–98 Ref
Mosquito density
 20 mosquitoes 194 148 46 76 64–79 0.13
 50 mosquitoes 480 410 70 85 79–87 Ref
 100 mosquitoes 927 696 231 75 67–74 < 0.01
Water bath temperature
 34 °C 330 271 59 82 51–95 0.23
 38 °C 335 299 36 89 81–100 Ref
 42 °C 320 277 43 86 72–98 0.61
 46 °C 305 244 61 79 58–96 0.09
  1. Ref: reference group for the calculation of the p values
  2. *Empirical averages were calculated as the average feeding rate of all replicates obtained for a specific condition
  3. **Significantly different from the reference when p < 0.05. Data were transformed using arcsine (p) prior to doing the paired t test