Feeding parameters | Total number of mosquitoes in cups | Total number fed | Total unfed | Empirical average feeding rate (%)* | Range (%) | p value** |
---|---|---|---|---|---|---|
Starving time | ||||||
 0 h | 916 | 240 | 676 | 27 | 4–45 | < 0.01 |
 2 h | 864 | 288 | 576 | 34 | 15–56 | < 0.01 |
 4 h | 933 | 368 | 565 | 40 | 20–54 | < 0.05 |
 6 h | 954 | 405 | 549 | 45 | 23–82 | < 0.01 |
 Overnight (~ 21 h) | 841 | 492 | 349 | 60 | 19–97 | Ref |
Type of starving | ||||||
 Access to water | 217 | 134 | 83 | 62 | 43–89 | 0.47 |
 Dry starving | 234 | 167 | 67 | 71 | 31–92 | Ref |
Membrane type | ||||||
 Baudruche | 476 | 389 | 87 | 85 | 70–100 |  < 0.05 |
 Parafilm | 457 | 268 | 189 | 53 | 42–76 | Ref |
Exposure time | ||||||
 10 min | 426 | 326 | 100 | 77 | 63–98 | 0.63 |
 20 min | 469 | 368 | 101 | 80 | 43–100 | 0.61 |
 30 min | 386 | 314 | 72 | 81 | 72–89 | Ref |
Mosquito age | ||||||
 3 days | 631 | 447 | 184 | 75 | 39–96 | 0.38 |
 5 days | 606 | 473 | 133 | 81 | 50–93 | Ref |
 7 days | 613 | 449 | 164 | 75 | 55–90 | 0.08 |
Light/Dark | ||||||
 Light | 317 | 274 | 43 | 85 | 64–96 | 0.88 |
 Dark | 326 | 276 | 50 | 84 | 63–96 | Ref |
Volume of blood | ||||||
 125 µL | 289 | 190 | 99 | 65 | 50–88 | < 0.05 |
 250 µL | 272 | 229 | 43 | 84 | 67–96 | 0.54 |
 500 µL | 295 | 256 | 39 | 87 | 77–98 | Ref |
Mosquito density | ||||||
 20 mosquitoes | 194 | 148 | 46 | 76 | 64–79 | 0.13 |
 50 mosquitoes | 480 | 410 | 70 | 85 | 79–87 | Ref |
 100 mosquitoes | 927 | 696 | 231 | 75 | 67–74 | < 0.01 |
Water bath temperature | ||||||
 34 °C | 330 | 271 | 59 | 82 | 51–95 | 0.23 |
 38 °C | 335 | 299 | 36 | 89 | 81–100 | Ref |
 42 °C | 320 | 277 | 43 | 86 | 72–98 | 0.61 |
 46 °C | 305 | 244 | 61 | 79 | 58–96 | 0.09 |