Fig. 2

MtnB.496 yeast facilitates scaled production of fit A. aegypti males. Larval consumption of MtnB.496 yeast does not significantly impact adult male mating capacity (a, P > 0.05, chi-squared test, n = 49), the number of eggs laid by females that mate with these males (b, P > 0.05, Student’s t-test), or the percentage of larvae hatching from these eggs (b, P > 0.05, Student’s t-test). Incorporation of MtnB.496 yeast into the diet used for mosquito mass rearing induced significant female mortality (c, ***P < 0.001 vs. mass-rearing diet, chi-squared test) yet did not significantly impact male survival (c, P > 0.05 vs. mass-rearing diet, chi-squared test; data were compiled from four replicate containers per condition, each bearing 500 larvae) or wing size (d, P > 0.05 vs. mass-rearing diet, t-test; n = 50 wings/treatment), which correlates with fitness. Error bars represent SEM in all panels. NSD: Not significantly different