Soil-transmitted helminth infections in free-ranging non-human primates from Cameroon and Gabon

Parasites & Vectors

Table 1 Primers and PCR cycling programmes

Target organism	Gene	Primer	Sequence (5'–3')	Product size (bp)	Thermal profile^a
					Step 1		Step 2		Step3		N
					Temperature (°C)	Duration (s)	Temperature (°C)	Duration (s)	Temperature (°C)	Duration (s)	N
Oesophagostomum/Necator	ITS2	NC1	ACGTCTGGTTCAGGGTTGTT	NA	94	30	50	30	72	45	45
		NC2	TTAGTTTCTTTTCCTCCGCT	NA	94	30	50	30	72	45	45
		OesophITS2	TGTRACACTGTTTGTC-GAAC	250/300	94	30	55	30	72	30	35
		NC2	TTAGTTTCTTTTCCTCCGCT	250/300	94	30	55	30	72	30	35
Trichuris trichiura	ITS2	ExtITS2	GGATCACTTGGCTGGTAG	NA	94	30	56	30	72	45	45
		NC2	TTAGTTTCTTTTCCTCCGCT	NA	94	30	56	30	72	45	45
		IntITS2	CTTGAATACTTTGAACGCACATTG	480–700	94	30	49	30	72	45	35
		NC2	TTAGTTTCTTTTCCTCCGCT	480–700	94	30	49	30	72	45	35
Ascaris lumbricoides	ITS1	ITS F1	CGAGCAGAAAAAAAAAAGTCTCC	NA	94	30	50	45	72	45	45
		ITS R1	GGAATGAACCCGATGGCGCAAT	NA	94	30	50	45	72	45	45
		ITS F2	CGAGCAGAAAAAAAAAAAAGTCTCC	~ 500	94	30	52	30	72	30	35
		ITS R2	GCTGCGTTCTTCATCGAT	~ 500	94	30	52	30	72	30	35

ITS Internal transcribed spacer, N number of cycles, NA data not available
All PCR analyses started with 95 °C for 15 min and finished with 72 °C for 10 min
^aStep 1: denaturation; Step 2: annealing; Step 3: elongation

ISSN: 1756-3305