Blastocystis in the faeces of children from six distant countries: prevalence, quantity, subtypes and the relation to the gut bacteriome

Table 4 Significant signals from DESeq2 testing of association between Blastocystis positivity and individual taxa within the stool bacteriome

Phylum	Class	Order	Family	Genus	Mean abundance of genus	Fold-diff (genus)	Corrected adjusted p value
↓ Actinobacteria	↓ Actinobacteria	↓ Bifidobacteriales	↓ Bifidobacteriaceae	*↓ Bifidobacterium*	6.0%	0.36	6.2 × 10^–6
Firmicutes	Negativicutes	Selenomonadales	Veillonellaceae	*↓ Veillonella*	1.3%	0.22	3.0 × 10^–4
	Bacilli	↓ Lactobacillales	↓ Streptococcaceae	*↓ Streptococcus*	1.5%	0.28	9.4 × 10^–7
	Bacilli	↓ Lactobacillales	↓ Lactobacillaceae	*↓ Lactobacillus*	1.3%	0.39	7.7 × 10^–3
	Clostridia	Clostridiales	Lachnospiraceae	*↓ Fusicatenibacter*	1.6%	0.45	1.0 × 10^–3
				*↓ Blautia*	7.9%	0.42	8.9 × 10^–8
				*↓ Anaerostipes*	1.6%	0.38	6.2 × 10^–6
			Ruminococcaceae	*↓ Subdoligranulum*	2.3%	0.48	7.9 × 10^–4
				*↑ Ruminococcaceae UCG-002*	1.5%	2.65	8.1 × 10^–5
				*↑ Ruminococcaceae UCG-014*	1.6%	4.16	7.7 × 10^–4

All models were adjusted for nationality; PCR positivity for Blastocystis was a dichotomous predictor
The model predictors were nationality and Blastocystis positivity in real-time PCR. The fold difference is the coefficient for the particular genus, associated with Blastocystis positivity. The P value comes from this adjusted DESeq2 model and is corrected for the number of tested taxa. Shown are significantly associated taxa with a mean abundance > 1%
Arrows and bold typeface indicate statistically significant associations

ISSN: 1756-3305