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Fig. 2 | Parasites & Vectors

Fig. 2

From: Giardia duodenalis extracellular vesicles regulate the proinflammatory immune response in mouse macrophages in vitro via the MAPK, AKT and NF-κB pathways

Fig. 2

p38 MAPK, ERK and Akt signaling pathway activation in response to GEV stimulation of mouse macrophages. a GEVs were inoculated into mouse macrophages, and cells were collected at the indicated time points for measurement of total protein and phosphorylated protein expression using western blotting. b Gray value analysis of phospho-p38/β-actin. c Gray value analysis of phospho-ERK/β-actin. d Gray value analysis of phospho-AKT/β-actin. The results are shown as the mean ± SEM of triplicate experiments. Significant differences vs 0-h control: *p < 0.05, **p < 0.01, ***p < 0.001

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