Fig. 2

p38 MAPK, ERK and Akt signaling pathway activation in response to GEV stimulation of mouse macrophages. a GEVs were inoculated into mouse macrophages, and cells were collected at the indicated time points for measurement of total protein and phosphorylated protein expression using western blotting. b Gray value analysis of phospho-p38/β-actin. c Gray value analysis of phospho-ERK/β-actin. d Gray value analysis of phospho-AKT/β-actin. The results are shown as the mean ± SEM of triplicate experiments. Significant differences vs 0-h control: *p < 0.05, **p < 0.01, ***p < 0.001