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Fig. 1 | Parasites & Vectors

Fig. 1

From: In vivo and in vitro efficacy of crocin against Echinococcus multilocularis

Fig. 1

ad Effects of crocin against Echinococcus multilocularis metacestodes in vitro. a Identification of metacestodes and germinal cells. E. multilocularis glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and mouse GAPDH were used for the characterization of E. multilocularis metacestodes and germinal cells (without host cell contamination). Lanes 1, 5 Metacestodes; lanes 2, 6 germinal cells; lanes 3, 7 mouse liver; lanes 4, 8 negative control. b Pathological observation of metacestode vesicles. An obvious germinal layer (GL) and a laminated layer (LL) were visible in metacestode sections after haematoxylin–eosin (HE) staining. Scale bar = 50 μm. c Metacestode sections were stained with periodic acid Schiff (PAS). The sections presented a strongly PAS-positive basophilic LL. Scale bar = 50 μm. d Effects of different concentrations of crocin against E. multilocularis metacestodes. The effects of a series of concentrations ranging from 0.5 to 160 μM on E. multilocularis metacestodes were tested by phosphoglucose isomerase (PGI) assay in triplicate. Triton X-100 (0.1%) served as a positive control; the result for the positive control group was set to 100%. The data are presented as the mean ± SD

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